Biotinylation of APEX2-tagged protein in the absence of H2O2 addition?

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Percy Zhang

It can happen when endogenous H202 catalyze the biotinylation. Sometimes apex2 is not required for biotinylation if the cell contains hemin.

That's why you need a negative control for your western blot and further MS. considering those proteins as background, and it will be easier for you to identify the desired protein.