I am doing an experiment to find out the best time and concentration for TMA-DPH. ( Three different concentration for different time points).
I would like to know how the result between the time and concentration should look like?
I want to know how to get result for this experiment and what I should keep in mind. It looks like an easy one but I did not get a perfect data for this experiment.
I tried to find papers that had done the same experiment on TMA-DPH but I could not find.
( I use CytoFLEX )
Thank you for your time and help.
Arwa
The relation is directly proportional and the rate is independent of reactant concentration, so the gradient is −k. Conversely, a graph of the concentration of any product as a function of time is a straight line with a slope of k, a positive value....
The relation is directly proportional and the rate is independent of reactant concentration, so the gradient is −k. Conversely, a graph of the concentration of any product as a function of time is a straight line with a slope of k, a positive value....
Thank for your answer.
I was trying to find the best concentration and time for my experiment. I have done 3 different concentration( 0.2 ul, 0.5 ul, 1 ul), in different time point ( 0, 5, 10, 15,30 mins).
I am not sure if 0.5 ul in 15 min is the best concentration and time?! If yes, why?
Why not 1 ul is the best concentration?
Thank you for your help.
Arwa
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