https://www.mdpi.com/journal/plants/special_issues/U4SF8Q6O0P
I am doing a systematic review, and I am measuring risk of bias with RoB2 for RCT, and ROBINS-I for non RCT. My questions is, for single arm studies, can I use ROBINS-I? I am not sure how to...
14 July 2024 3,570 5 View
I've been working with activated sludge samples and have encountered a challenge in identifying certain structures/organisms that appear abundantly within the flocs during optical microscopy...
23 June 2024 9,584 1 View
I use ELISAs for quantification of extracellular proteins in cell-conditioned media. I am interested in quantifying uncoupling protein 1 (UCP1) which is an intracellular, mitochondrial...
27 May 2024 8,025 1 View
Can Sensory marketing affects customers satisfaction in Hotels?
12 May 2024 3,308 3 View
Hello everyone, I would greatly appreciate it if someone could assist me in understanding the steps required to estimate the sample size for a longitudinal study. Or advise bibliography. I am...
04 April 2024 2,882 0 View
I am using mouse serum to block Fc receptors before staining, for flow cytometry. My question is if I should also add the serum to compensation beads before staining them, so that the cells and...
07 March 2024 9,989 2 View
Most staining protocols for flow cytometry in 96-well plates use V-shape or U-shape plates. I would like to ask if staining could also be done in flat-bottom plates. Thank you!
07 January 2024 2,910 5 View
I plan to run my experimental samples in the flow cytometer in batches. My question is if FMO controls have to be prepared and run every time I run experimental samples, or if I could only do them...
04 January 2024 7,842 0 View
I am immunophenotyping PBMCs by flow cytometry using markers for T cells to analyse the different subsets (Naive, CM, EM and EMRA), and to measure senescent and exhausted t cells. I want to...
02 January 2024 5,387 1 View
Hi, we've been unsuccessful in creating single clones with deletion of a couple of proteins, after testing several sgRNA sequences for each. We are using a lentiviral vector encoding puromycin for...
25 December 2023 2,901 2 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
Now research fellow used it to prevent fungus in potato,may be potatoes gene effect.
02 August 2024 180 3 View
Please discuss someone told about fungus and allied others.
31 July 2024 3,372 3 View
Now i am working on AM fungi single strain. What are the most effective isolation techniques for obtaining pure cultures of arbuscular mycorrhizal (AM) fungi?? Is there are best approach...
26 July 2024 6,520 1 View
How can we measure their colony diameter?
12 July 2024 7,027 0 View
I am carrying out an experiment on Trichoderma and need to measure OD of the fungal growth after 7 days. Unlike bacteria, fungus clump together making It difficult to get OD for the fungal growth....
11 July 2024 6,251 1 View
We have been experiencing repeated what looks very much like fungal contaminations in several incubators (in the same lab). We have tried the following repeated times and yet the coating on all...
02 July 2024 6,375 2 View
..?
15 June 2024 6,072 2 View
I have been trying to culture P. falciparum, but I see contamination after ~1 week of culturing. What could this organism possibly be (image attached)? The culture media consists of RPMI, HEPES,...
15 June 2024 5,515 5 View
Hola a todos. Me gustaría saber si alguien conoce algún documento de consulta de claves dicotomicas - taxonómica para hongos microscópicos, especialmente que me pueda ayudar a determinar especies...
15 June 2024 6,693 0 View