I have tested to extract RNA from small volumes of human red blood cells and as well-known, they do not contain much RNA. I need to get RNA with high RIN-values (RNA integrity) and purity and so far TRIZOL-LS extraction followed by RNeasy minelute clean up kit, gave to low yield and not good enough RNA-quality. I have also tested RNeasy Mini kit with some modifications with varying results, depending on the blood samples and since we are working with rare blood groups the collection of RNA-samples must be performed with a solid method. I want to perform RNA-seq on the samples later on. Any suggestions for a better RNA-extraction method for RBCs?
Thanks. Did you clean up your RNA-samples on a column (RNeasy column or other) afterwards, to enhance the purity of the RNA?
Thanks a lot for your valuble information. I will try and see it it works in my hands as well.
Thank you again.
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