Best way to calculate concetration of radiolabeled RNA?

04 April 2018 1 9K Report

Is there a good way to determine the concentration of radiolabeled RNA say after radiolabeling an RNA, ligation to another piece, and then looking at the CPM after gel excision and extraction?

Abdallah Hussien Fathy

Look at the calculations in the following links

https://pdfs.semanticscholar.org/7ed8/d41eb77b33c9feb4f479c10c7931257073ca.pdf

https://assets.fishersci.com/TFS-Assets/LSG/manuals/MAN0011905_Syn_Radiolabel_RNA_Probes_HiSpecActiv_UG.pdf

https://www.thermofisher.com/us/en/home/references/ambion-tech-support/nuclease-enzymes/tech-notes/determining-rna-probe-specific-activity-and-yield.html

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