Kurtis Breger

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Questions related from Kurtis Breger

Is there an optimal amount or range of BSA to add to enzyme reactions?

I was wondering if there was a standard amount of BSA that one might add to an enzyme reaction or if there is a range one has to test out to find out if there are any improvements to enzyme...

12 December 2018 6,150 2 View

Anyone seen Nuclease P1 produce a third spot on TLC when looking at Adenosine and its modifications e.g. m6A?

I've been seeing a third spot on my TLC near the solvent front with a phosphorimager. I am digesting nucleotides with Nuclease P1 to observe adenosine and N6-methyladenosine. I believe it is...

07 July 2018 8,328 0 View

Best way to calculate concetration of radiolabeled RNA?

Is there a good way to determine the concentration of radiolabeled RNA say after radiolabeling an RNA, ligation to another piece, and then looking at the CPM after gel excision and extraction?

04 April 2018 8,977 1 View

What are some ways to increase the yield of multi-RNA splint ligation?

I am currently having some issues getting a good yield for my three-way splint ligation. I am using a DNA splint that covers the entire length of all three oligos. I get some full length RNA, but...

03 March 2018 9,823 2 View