I've tried the Qiagen RNeasy Mini kit on chicken blood samples but got no RNA.
Started with 1ml fresh whole blood collected on ETDA, after adding the 600ul lysis buffer the lysate was SOOO VISCOSE, and so hard to pipette even after trying to homogenate it with "Syringe & needle 20 guage co.9 mm"
Knowing that the whole blood of birds, reptiles, and amphibians contains large quantities of protein that must be separated from the nucleic acids to perform a successful extraction, But I don't know how ?!
- should I start with less quantity of blood or what should I do
- the pic shows the pellet size before I discard the supernatant & adding the lysis buffer
I think 1 ml whole blood is too much, for 600 ul lysis buffer I would not exceed 100 ul of the whole blood.
I agree with the previous response, using too much rarely results in a good, clean, sample.
If that doesn't work and you still want an alternative kit, try Zymogen products. I always got great results, and I believe they will send a free sample.
The avian blood is very concentrated, so it would be better to study with low volume of blood. Remind that avian erythrocytes contain nucleus. So, I also suggest you to dilute the blood samples with RNAse/DNAse free water before isolation. You can find enclosed protocol. Good luck!
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