Hi,
I am currently working on optimizing the protocol in my lab for 2P imaging using a water dipping objective. I'm using mice with implanted GRIN lenses that are 0.6x7.3mm, imaging amygdala neurons (injected at approx -4.7mm depth from bregma). I'm having trouble maintaining the meniscus between the objective and the lens. Are there other compounds that people use or any methods to maintain the meniscus that I may be unaware of? Thanks for any advice!
Hi,
I am not sure if you tried that, but what we did was 1. create a "well" using dental cement around our window 2. on the edges of the dental cement well we applied a thin layer of vaseline to keep the water within the well. Just a tip: you should clean the objective between mice because vaseline might stay on the objective.
Good luck!
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