Best online tools for TaqMan probes and primer design ?

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Sharif Hasan Siddiqui

https://www.thermofisher.com/taqman/gene-expression/assay/query?keyword=ITGAM&productType=ge&productSubtype=ge

Hi,

You can try this link. It might help you.

Yoav Lubelsky

https://eu.idtdna.com/scitools/Applications/RealTimePCR/

https://www.ncbi.nlm.nih.gov/tools/primer-blast/index.cgi?LINK_LOC=BlastDescAd

https://www.primer3plus.com/index.html

Stacy Colaco

Thanks Sharif and Yoav, I'll try these revert!

Nandaraj Taye

Hello, here is a step by step video tutorial on primer designing using ncbi primer blast. https://www.youtube.com/watch?v=wGRhN4rWA2Q

Hi all, I found that IDT PrimerQuest worked best for me. This tool not only predicted Taqman primer and probe combinations based on my given template but also provided links to assess self complementarity and primer blast.

Thank you everyone.