Has anyone had problems transforming the pSATN BiFC vectors (doi:10.1016/j.jmb.2006.08.017) into the Agrobacterium strain GV3101?

Hi! I've been having problems transforming clones I made using the pSATN BiFC Gateway vectors generated by Tzvi Tzfira's group (doi:10.1016/j.jmb.2006.08.017), which I purchased from the...

12 February 2020 9,574 3 View

I'm looking for resources on how to use NVivo for analyzing data using Heideggerian hermeneutic phenomenology?

I am currently working on my dissertation and using NVivo to analyze my data. I started line by line hand coding and decided it was going to take too long, so I started using NVivo. I am going...

08 March 2019 2,482 3 View

Does anyone have experience with low-input kits for RNASeq library prep following unexpectedly low RNA extraction yields?

21 April 2016 1,231 1 View

Does anyone have experience with H9 Cre-LoxP cell line?

10 August 2014 2,588 3 View

How to interpret codeml output from PAML (Phylogenetic Analysis by Maximum Likelihood)?

I simply want one omega value (dn/ds) for a multiple sequence alignment. I am using CDS sequences that have been codon aligned and are from 19 different strains of the same species (so the...

29 May 2013 1,259 7 View

Calculating dN/dS from bwa assembly

I have taken Illumina reads and aligned them to a reference genome using BWA then obtained the corresponding BAM/SAM files. I have also called SNPs, which are in VCF format, and tried to use this...

02 April 2013 3,660 9 View

The nature of a police institution

What is the nature of a police institution - civil or militarized? The question is related to democratic countries.

06 September 2012 1,002 3 View

Project based learning ideas for first year biology majors - Animal Behavior and Conservation Biology

In search of assessment alternatives to exams as I now teaching online. Appreciate this supportive community and wanted to reach out. TIA.

01 January 1970 5,431 3 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View