Respected Mariana Colaço

To aliquot polymyxin B sulfate salt (P4932-1 MU) from Sigma-Aldrich, begin by preparing a stock solution of the desired concentration, following the instructions provided by Sigma-Aldrich or referencing the product datasheet. Once the stock solution is prepared, ensure proper sterilization techniques are employed to maintain purity and prevent contamination. Using an aseptic technique, dispense the desired volume of the stock solution into sterile aliquot tubes, taking care to avoid introducing air bubbles. Seal the aliquot tubes securely to prevent evaporation or contamination during storage. Label each aliquot tube with the date of preparation, concentration, and other relevant information. Store the aliquots at the recommended temperature and conditions specified by Sigma-Aldrich to maintain stability and functionality.