Has anyone had problems transforming the pSATN BiFC vectors (doi:10.1016/j.jmb.2006.08.017) into the Agrobacterium strain GV3101?

Hi! I've been having problems transforming clones I made using the pSATN BiFC Gateway vectors generated by Tzvi Tzfira's group (doi:10.1016/j.jmb.2006.08.017), which I purchased from the...

12 February 2020 9,574 3 View

I'm looking for resources on how to use NVivo for analyzing data using Heideggerian hermeneutic phenomenology?

I am currently working on my dissertation and using NVivo to analyze my data. I started line by line hand coding and decided it was going to take too long, so I started using NVivo. I am going...

08 March 2019 2,482 3 View

Does anyone have experience with low-input kits for RNASeq library prep following unexpectedly low RNA extraction yields?

21 April 2016 1,231 1 View

Can anybody recommend a public database suitable for validation data for a specialised psychometric instrument?

12 August 2015 6,114 3 View

Why are plots of PCA factor loadings so different to MDS plots of the same data?

PCA and MDS both do a similar job in the sense that they extract underlying dimensions or components from data, so why are plots of PCA factor loadings often so different to those yielded by MDS?

23 February 2015 6,453 3 View

Does anyone have experience with H9 Cre-LoxP cell line?

10 August 2014 2,588 3 View

How to interpret codeml output from PAML (Phylogenetic Analysis by Maximum Likelihood)?

I simply want one omega value (dn/ds) for a multiple sequence alignment. I am using CDS sequences that have been codon aligned and are from 19 different strains of the same species (so the...

29 May 2013 1,259 7 View

Calculating dN/dS from bwa assembly

I have taken Illumina reads and aligned them to a reference genome using BWA then obtained the corresponding BAM/SAM files. I have also called SNPs, which are in VCF format, and tried to use this...

02 April 2013 3,660 9 View

Project based learning ideas for first year biology majors - Animal Behavior and Conservation Biology

In search of assessment alternatives to exams as I now teaching online. Appreciate this supportive community and wanted to reach out. TIA.

01 January 1970 5,431 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

Is there a classification scheme for rock cavities or caverns with respect to their size and shape?

The range of forms and sizes of cavities in rock is very big. Is there a classification scheme for rock cavities regarding their size and shape?

02 March 2021 4,511 3 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

Is there a deep reason for using the squared modulus of the wave function as the probability density of the existence of the particle?

I think we were all surprised at the first time we got to know quantum mechanics that the squared modulus of the wave function is the probability density of the existence of the particle? The role...

28 February 2021 6,282 9 View

How the wettability of rock sample can be changed by ageing in pure hydrocarbon oils only?

I want to do wettability analysis of hydrocarbon oil (octane)/rock/surfactant solution system. but to do the analysis first I have to make rock surface oil-wet. Since hydrocarbon oil does not have...

27 February 2021 5,383 3 View

What could be reason for getting such type of graph for complex impedance in case of polymer blend films?

Its effect appears in real part of ac conductivity also. Can anyone suggest any reason behind it?

26 February 2021 3,487 3 View

Any references about insects or animals that can damage or weathering of rock or fossil material?

Are there any references to insects or animals that can cause damage or weathering to the rock or fossil material? books or paper that would be nice. Thank you very much!

24 February 2021 6,805 3 View

Physical-chemical formation of stalactites/stalagmites. what promotes the formation/growth of these structures?

I know, or think I do, the basic idea behind these natural structures, as from what I know they form due to water dripping from the top leaving a salt deposit behind. But started to wonder about...

22 February 2021 1,400 4 View

Resistivity of PVD TiNx thin films vs. N2 flow?

In the PVD of TiN thin films (~100A), I've found the resistivity of the TiN films dropped from ~100 to 60um*cm with N2 flow increasing up to the metallic mode ending point; then the resistivity...

21 February 2021 1,864 2 View