Hello, Ebony!

Please You look at the article:

Article Targeted Overexpression of α-Synuclein by rAAV2/1 Vectors In...

Recombinant adeno-associated virus 2/1 preparation and cell culture

Animals and surgical procedures

Hi, for almost all in vitro experiments using AAV I'd recommend AAV-DJ. Is this an immortal cell line or primary cells? If they are immortal then lentivirus is probably a better option as AAV will be lost on cell division. However, some mouse cells are resistant to LV infection. I can see you're at UCSF, the Stanford GVVC vector core used to offer test 'stock' viruses when I worked there. You can try some different serotypes or LV promoters inexpensively and see if anything works

Dear Ebony,

I have tried AAV and lentiviral expression of alpha-synuclein in cultured neurons and both work well. If you want to infect all the neurons in the culture, I would use AAVs (e.g. serotype PHP.B). However, if you want to control the number of infected neurons I would recommend lentivirus, because you can adjust the titer more easily. For lentivirus expression I have used the classical FUGW backbone (ubiquitin promoter).

Hope this helps,

best wishes,

Chris