Does anyone have a well established formula for Klotz solution? I found 2 online but one has sodium sulfate and the other does not. Thank you.
I am using a BD FACSMelody which currently has three lasers and 8 channels. When designing panels, I'm concerned about the spectral overlap between different fluorophores in some of the channels....
12 December 2022 5,828 1 View
Seeking advice from Salina KS, USA: I am in desperate need of a UV trap to live-trap and monitor (NOT kill) night-flying moths. Here are the requirements: -- Has to run off a battery for 12 hrs....
23 June 2021 8,096 3 View
Hello, I am trying to induce the over-expression of alpha synuclein in mouse neuron cultures. I want to try to see whether a specific AAV or Lentivirus would help me induce over expression. Has...
24 April 2021 3,817 3 View
I conducted a greenhouse experiment recently that involved rearing corn plants in a calcined clay medium (Turface) and Long Ashton solution. Despite fertilization with Long Ashton 3 times a week,...
16 March 2017 2,810 12 View
I would like to stain surface, intracellular cytokines, and nuclear transcription factors on the same samples if at all possible. I have been cautioned that the aggressive detergents needed to...
15 February 2017 7,935 2 View
Would it be better to use cDNA or genomic DNA to run a conventional PCR on tissue from a goat?
08 June 2016 8,953 6 View
Does anyone know where I can find water-soluble testosterone suitable for cell culture? Sigma has discontinued theirs and I have been unsuccessful in finding another. Can water-insoluble...
28 July 2014 9,567 15 View
My lab is interested in conducting an insect bioassay on phytochemicals from an uncommonly studied plant species, Silphium integrifolium. We are looking for a simple protocol we can use to...
01 January 1970 7,700 4 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...
03 August 2024 9,918 0 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
I am planning to prepare a decellularized porcine tissue derived biomaterial. It would be very helpful if I get to know the composition of the solution which is ideal to bring the tissue sample...
31 July 2024 5,645 2 View
I'm new to primary human fibroblast culture and I'm studying aging using cells from old and young donors. Some research groups have used media supplemented with FGF2, so I cultured cells in the...
29 July 2024 3,030 2 View
My question pertaining to the DAB staining in cytoplasm of human oral squamous cell carcinoma tissue. When quantifying the epithelial cancer cells do we have to crop remove the stromal tissue?...
29 July 2024 2,682 6 View
I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.
29 July 2024 8,560 0 View
Hello everyone, A collaborator sent us his protocol in order to be able to use his immunohistochemistry kits. But one step contains PBS + 0.3% H2O2 + 0.1% sodium azide. Knowing the dangerousness...
23 July 2024 6,314 3 View