I'm planning to make a lot of dsDNA for a fair number of targets (200-800bp in size), using the T7 cloning method. I was wondering if anyone had any opinions/advice for once the dsRNA is reverse translated how to time and cost-effectively purify it with a good recovery rate. Also, does anyone know if dsRNA is more chemically similar to DNA or RNA in regards to its interaction properties with column materials?
You could use anion exchange columns. Check these documents, please
https://rnajournal.cshlp.org/content/12/5/807.full Article High-throughput purification of double-stranded RNA molecule...
You could also follow this researchgate link: https://www.researchgate.net/post/How_to_purify_dsRNA_produced_by_E_coli2
Don't use columns since you will not be able to recover the full amount of dsRNA. Instead, after the T7 transcription reaction, purify it with acid phenol/chloroform and precipitate it with ethanol and sodium acetate.
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