Hi, I have a lot of videos (4x 24 hours in 2 min bins, for ~40 animals. Animals recorded in groups of 4 ) which I need to analyse circadian activity from. I have tried the automated tracking programs however I have been limited by my computers RAM (deeplabcut just crashes) and the noise in the videos (the animals are relatively well camouflaged so processing to isolate doesn't really work.)

The only way I have had any luck is using manual tracking in the imageJ TrackMate plugin.

Are there any other program which are typically used for manual tracking of activity? TrackMate gives me most of the information I need however I just want to check if anyone has any better recommendations I might have missed.

I've tried (with no luck):

-Bonsai

-Deeplabcut

-Animal tracking (imageJ plugin)

-ethowatcher

-ethovision

-Manual Track in imageJ

-TrackMate

-A couple of the less common automated ones which I forget the name of!

I'm completely happy to do manual tracking so not after suggestions for automated/ semi-automated tracking software! Due to the housing of the animals semi-automated does not work. Also needs to be opensource!

Thanks a million in advance!