I would like to express a GECI in primary epithelial cells. I Would like to get some suggestions for to chose the best GECI reported. Are there some GECIs reported such as GCaMP5G, GCaMP6s, GECOs, but I don't know what is better than other.
Thanks in advance.
Since you're measuring in epithelial cells, I assume you do not need to measure fast dynamics with your Ca2+ indicator. Therefore, I'd recommend using a high affinity GECI. This would include the "s" (slow) forms of GCaMP, which tend to have around a 1 second decay time (related to the rate of unbinding Ca2+). GCaMPs are also the best characterized. There are new and improved GCaMP versions out there that tend to be sensitive and fast (8, etc) but 6 is tried and true and gets the job done. Note this would occupy your green channel, so select other fluorescent makers wisely.
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