Based on the TCL results, can predict the flavonoid/alkaloid or any compounds present in the extract of Vernonia amygdalina Del.?

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Ikram Madani Ahmed

Article A useful spray reagent to differentiate common phenolic comp...

Your plates show no clear spots. Try to spray reagents. Phenolics and alkaloids have different spray reagents, Try the reagent used in the above article.

Aprajita Tiwari Pandey

As per my observation the solvent system you used does not show proper separation of your secondary metabolites.Hence I suggest you to standardize your solvent system and for detection of Favanoid/alkaloid go with HPTLC.

For standardization TLC and HPLTC a reference paper is attached under:

https://www.researchgate.net/publication/351009179_Validating_Anti-Infective_Activity_of_Pleurotus_Opuntiae_via_Standardization_of_Its_Bioactive_Mycoconstituents_through_Multimodal_Biochemical_Approach

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