Hello Geetha V C

You may try these.

1. Sas6, which is a protein that plays a crucial role in the formation of the basal body, a structure at the base of the primary cilia. It is specifically involved in the assembly of the cartwheel, a hub-and-spoke structure that organizes the nine-fold symmetry of the basal body.

You may use antibodies specific to SAS6 to visualize its location within the cell, particularly within the centrioles and basal bodies, which are precursors to cilia. Techniques like Western Blotting may be used to confirm the presence and abundance of SAS6 protein.

2. IFT (intra flagellar transport) proteins which are involved in transport within the cilium and also play a role in basal body function and maintenance. To identify IFT proteins within cilia, you may use live-cell imaging with fluorescently tagged IFT proteins which will allow for visualization of their movement and localization within the cilium.

Best,