23 March 2024 0 9K Report

I have been trying to identify some fungal samples using ITS primers and Beta tubulin primes. I got one species from ITS as the top hit but the same species name comes as the second or third hit from beta tubulin primers even though I have used the same sample (DNA extraction). Is it ok to choose that second or third hit from the beta tubulin primers to identify the fungal sample? The matching scores are also between 98- 100.

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