11 Questions 7 Answers 0 Followers
Questions related from Geetha V C
Hi, kindly suggest me some basal body specific markers in primary Cilia other than Gamma tubulin ?
20 June 2025 3,653 1 View
I tried to amplify my GOI from genomic dna as the template. I did a gradient PCR for the standardization of the primers . All I got was a smear. I couldn't figure out what's the problem here ! I...
12 June 2025 9,183 10 View
Suggest a better software for pegrna and sgrna design. I'm trying to use docker and so on as per how it's mentioned in GitHub to use Primedesign by pinellolab. I could not get CFD score in this....
30 May 2025 3,168 0 View
Which is the appropriate cell line to study cilia in mice? I want to do a primary cell culture from mice. Suggest me choose which organ/tissue in mice should I prefer to study ciliary molecules.
27 May 2025 6,555 2 View
Can anyone tell the list of restriction enzymes that can possibly be used for cloning of the minigene construct in pspl3 vector. I could not find an appropriate sequence map that tells me the...
21 May 2025 9,858 1 View
Suggest things I need to keep in mind while doing a KO in htert rpe-1 with aio-mcherry plasmid. ( I know the overall workflow , hope to get technical suggestions to be cautious )
16 May 2025 2,650 1 View
since htert rpe-1 are adherent cells , the only additional step I do is trypsinize pellet with dmem and then proceed with trizol lysis. Is this the step that's giving me problems in rna isolation?...
29 April 2025 1,474 1 View
Hi, Can anyone tell what's the minimum \ maximum dna concentration that can be used for the transfection of htert rpe-1 cells. And also we suddenly ran out of optimem. So I used 2% dmem-f12 as...
15 April 2025 9,158 3 View
InI m working with htert rpe -1 cell line for cilia visualization. I want to see whether my gene of interest is affecting cilia morphology. My GOI (693bp) is cloned in p3x vector ( 6299bp ) which...
07 April 2025 3,257 1 View
What can go wrong because of an increased cilia length ? is there a way I can assay that? My second question is whether there is any technique known or designed to assay ciliogenesis defects ? I...
01 January 1970 6,357 0 View
Hi, I want to do prime editing in htert rpe-1 cells. I want to choose a prime editing vector. I would prefer a vector where we can clone both pegRNA and nicking sgRNA. MLH1-dn should be there. I...
01 January 1970 2,788 0 View
