BAL fluid desalting and concentrating with AMICON kit - which exchange buffer do you use?

02 June 2022 0 2K Report

Hi,

I want to desalt and concentrate bronchoalveolar lavage fluid (BAL) and have the Amicon kits.

Does anyone know which exchange buffer to use?

This paper Article Protein composition of bronchoalveolar lavage fluid and airw...

Bartlett et al. (2013), where they exchanged BAL against 100 mM tetraethlyammonium bicarbonate, although this was in pigs.

Lots of papers use the Amicon

https://www.nature.com/articles/s41598-020-69281-8

Can anyone help?

Thanks!!

Puja

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