Bacterial RNA extraction

More Daniel Weng's questions See All

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

Which database would give me this list of chemical components?

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When I mix oil and NaOH solution, Why solid layer in the surface is formed?

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22 February 2021 2,116 4 View

Rearranging abbreviation list in LaTeX ?

Hi, I'm trying to rearrange an abbreviation list for a manuscript such that it would be listed in alphabetical order. Is there a function that does this automatically or is manual my only option?...

18 February 2021 4,870 2 View

Person-Codes for questionnaires in longitudinal studies: Is there an easy solution for better matching-rates?

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17 February 2021 3,075 4 View

How to fix weka classification errors?

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08 February 2021 5,900 6 View

How to measure the concentration of a labeled protein with no tryptophan or tyrosines?

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04 February 2021 4,482 4 View

Is the use of "multivariate " or "multivariable" was correct in multiple responses study ?

In statistics most of the time, we use these two-term exchangeable, but in my opinion, there is a difference between them. Please suggest your opinion

04 February 2021 7,407 7 View

How do microbes tell the presence of an inorganic minaral in the soil?

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03 February 2021 4,630 10 View

Do you have any suggestion for multicriteria decision-making software?

I want to compare a set of alternatives by using multicriteria assessment but testing both compensatory and non-compensatory decision rules. At the moment I have in mind Definite...

25 January 2021 2,454 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

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03 March 2021 3,568 1 View

How to increase a model accuracy ?

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Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

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Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

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26 February 2021 6,799 3 View

Calculating volume of DNA required from DNA concentration?

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26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

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25 February 2021 6,969 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

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25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

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25 February 2021 3,612 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

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24 February 2021 5,749 3 View

Aamal Ghazi Mahdi Al-Saadi

Here is the protocol that I used to extract bacterial RNA from tissue culture

http://emerald.tufts.edu/~mcourt01/Documents/Trizol_protocol.pdf

Good luck

Arvind Singh Chandel

you can follow these references

1. Chomczynski, P., and Sacchi, N. (1987) Anal. Biochem. 162, 156.

2. Chomczynski, P. (1993) Biotechniques 15, 532.

3. Ausubel, F.M., et.al, eds. (1990) Current Protocols in Molecular Biology, Vol.2, Greene

Publishing Assoc. and Wiley-Interscience, New York, p.A.1.5.

4. Simms, D., Cizdziel, P.E., Chomczynski, P. (1993) FOCUS® 15, 99.

5. Bracete, A.M., Fox, D.K., and Simms, D. (1998) FOCUS 20, 82).