Is it important to count the number of bactria in wastewater sludge before inoculati?
Hello Fatemah,
The bacterial count would affect the growth of inoculated bacteria. Hence a pretreatment can be helpful. Are you using any pretreatment before inoculation?
Yes. Bacterial quantification is required to get idea of bacterial population and to maintain optimum food to bacteria ratio.
Wastewater sludge is not formed by bacteria only. Protozoa play very impostant role. And an activated sludge in a wastewater treatment plant treating industrial wastewater contaning phenol is formed mostly by yeasts. I suppose you have to look for porformance of thge whole wastewater sludge, not for bacteria only.
Best regards
Vit
Hello Karishma Shah,
There is not any pretreatment, just purging with N2 to remove all O2.
Is it enough to mention the volume of the wastewater used without quantification?
Bets regards
For wastewater sludge, is there certain range of bacterial count and diversity?
Hello Dr. Deepak,
Yes, you are right, however, some publications did not mentioned the bacterial count in wastewater sludge before MFC inoculation (as below),
Does this affect the possibility of experiment repetition?
If we assume that there is certain range of bacterial number and/or the bacterial cells are numerous, there is no need for quantification, and the repetition of the experiment is possible.
What do you think?
Regards
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Hello Fatemah,
I would also recommend Vit Mateju's suggestion as it is not just about bacteria present that could affect so keeping in mind to have an accurate research overall quantitative analysis is necessary to be done.
Vit Mateju and Karishma Shah Thank you for your reply.
I discussed the possibility of bacterial quantification with MFC expert and the answer is that it is difficult to quantify bacteria in wastewater sludge as it contains a huge number of bacteria, so it is of no problem if the sludge is used as inoculum without quantifying.
In my opinion quantifying bacteria in wastewater sludge inoculum is similar to quantifying the infinity, it appears non logical to count.
However, in case of diversity studies it is important to know as many as possible the microorganisms types in the wastewater samples.
Best regards
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