I am working to purify and determine the structure of a membrane protein with post transnational modification such as glycosylation. So, can anyone suggest any bacterial strain (and/or reference paper) enabling post translational modification.
To my knowledge, there is no bacterial host that will guarantee post-translational modification similar to the glycosylation pattern found in eukaryotic proteins. Thus, if glycosylation is important to you, it would be preferable to use a eukaryotic expression system, such as insect cells or Leishmania tarentolae (the latter available from Jena Biosciences
PTMs are common in eukaryotic proteins and are rare in bacteria. Although the production of glycoproteins has been seen as a virulence factor in pathogenic bacteria, the use of pathogens as a recombinant protein production system is not allowed! In some cases, the metabolic engineering of bacteria could produce glycoproteins.
Due to the above, eukaryotic hosts usually used for the industrial production of glycoproteins.
I've already seen articles, that yeasts (especially Pichia pastoris) are used to produce membrane proteins. Find an example here:
Article Large-Scale Production of Membrane Proteins in Pichia pastor...
Bacteria don't glycosylate much (typically only minor O-glycosylation). You need a golgi to get N-glycosylation (Yeast or higher cell types).
You should try yeast expression system like Pichia to get proper PTM. you will never get it in bacteria
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