Is it possible to extract bacterial genomic DNA using GenElute Plant Genomic DNA Miniprep kit from Sigma? Has anyone tried it? I have this kit and want to use it.
EHR/EMR is a document (in electronic format) on the health status of the patient, on preventive measures implemented, on treatment, medicines and recommendations related to health maintenance. The...
20 February 2024 1,015 4 View
In the first picture we have two smith diagrams for two antennas that are in resonance. I wonder why one circle is bigger and one smaller? Also, in the second picture, if someone can explain to me...
10 September 2023 8,145 3 View
What does parameter S21, the first quadrant in the picture, mean when it says lm -5.61? the picture shows two examples, one at an antenna distance of 75 cm and the other at 125 cm.
08 September 2023 8,795 3 View
Hello, we had a problem during our Pyrosequencing (at Pyro Q24 analyser). Some of them are: 1) Methylation CTRL at 30% (instead of 100%) at all 5 CpG sites 2) unmetylated CTRL giving identical...
11 May 2022 9,600 3 View
There are a number of ICT applications in health care practice today ( hospitals, polyclinics, general practice, nursing, public health etc.). Evaluation and improvement of such application is...
15 November 2020 3,339 2 View
I am working on microencapsulation of gallic acid with sodium alginate and when I dissolve beads in sodium citrate I have no antioxidant activity. Because of that, I was wondering how sodium...
13 October 2019 1,416 0 View
I am interested in the interpretation
25 July 2013 5,111 6 View
What should be preferred in standardization of EHR ?
25 December 2012 7,763 9 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...
01 August 2024 1,514 1 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
Hi, researchers, Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?
29 July 2024 4,570 0 View