I want to capture bacteria with Carbon dots, but i get flourescence intensity like jumping up and down by UV, and then i am confuse, why like that? or may be i need to measure OD 600 and Flourescence intensity without carbon dots, because i know E.coli did not have fluorescence, so i want to make sure with do that. and now i get Florescence of e. coli when dissolve in Phosphate buffer and also DI water, the problem is the flourescence still jumping up and down from the higher concentration (10^9) until the lower concentration?
E. coli bacteria do have fluorescence, which comes from such things as flavins, NADH and NADPH. They also scatter light, which can appear as fluorescence, and can also cause unstable fluorescence readings as they settle out of a suspension.
Thanks Adam B Shapiro, so how to solve this problem? i need to use E. coli and i want to try to capture my carbon dots with this bacteria because before when i try to capture E.coli, the fluorescence still jumping up and down
The longer the wavelengths used, the less interference you will get from the bacteria. UV will be much worse than red.
Perhaps you can use confocal fluorescence microscopy, enabling you to see individual bacteria and quantum dots.
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