Hi All,
I have B16F10 cell lines that I kept them in (-80) for about 2 weeks before I transferd them to liquid nitrogen , is it ok to still use these cell lines ? because i have read that ususally people keep them (24-48) hrs at -80 then from there they transfer them to liquid nitrogen, so I am not sure that two weeks at (-80) how much would affect on these cell lines and their behaviour? let me know if anyone have an idea about that
Subhash C. Juneja
They are perfect. Even I have kept cell lines at -80 alone for a year and doing now-a-days at -80C, they work perfectly. No worry on this issue of transferring to LN2 after 2 weeks at -80C,
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Muthanna Ali Sultan
Thank you so much for your answer Mr.Juneia, I will go a head and start working with them.
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