Azide inhibition of hrp?

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Vince Murphy

The azide concentration in your primary buffer should not interfer with your HRP tagged secondary antibody as it will be removed in the washing step.

Sarah Hopson

Thank you. I'm having trouble with the blot signal and am trying to think of possible reasons.

Nasir Abbas

@ Vince Murphy

could you give your opinion using sodium azide buffered primary antibody when in case of paraffin section?

would it interfere with HRP conjugated secondary Ab? How to avoid the possibility of interference? thanks in advance