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Hi, I am doing a gel shift assay to confirm that my recombinant protein will bind to DNA in vitro. I am using my rBAF180 and miniprepped pET30 vectors. I have tried undigested and digested...
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I am trying to conduct a western blot. My primary antibody is in a buffer with 0.09% sodium azide. Would this amount of azide be enough to inhibit HRP?
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The protein I am studying is mutated often in cancer. Researchers often mention LOH. So I have two questions: 1. Does loss of heterozygosity automatically mean that no protein is expressed? 2....
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