Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Agarose gel purification pre MinION sequencing?

Dear all, we performed an agarose gel purification with MinElute kit (QIAGEN) to extract bands between 3500 bp and 6000 bp for further sequencing with MinION. After the purification process,...

16 February 2021 5,178 1 View

How many samples can be pooled together for NGS?

We collected faecal samples from various species that we want to analyse their microbial communities' DNA via Next-Generation Sequencing. The problem is that due to budgetary constraints, we will...

16 February 2021 5,466 4 View

Does anyone recommend an antibody for cAMP detection in mouse brain tissue (WB or IHC)?

21 April 2020 6,336 0 View

Is there any alternative to a fume hood when working with DTT?

I am going to work with dithiothreitol (DTT) whilst isolating DNA and RNA, so I was looking for a safe alternative to a fume hood, such as maybe a specific type of mask or anything different that...

17 February 2020 3,656 3 View

How can I concentrate peptides?

I am working with an initial volume of 20 mL* and using a 10 kDa filter to separate proteins from peptides. With this filter I am able to concentrate the proteins upstream of the membrane....

15 January 2020 1,520 7 View

Why is one plasmid not transfecting HEK 293t cells , while a simliar second plasmid sucessfully transfects the cells with the same protocol?

I ordered and produced a plasmid from Addgene containing PIK3CA and GFP (pHAGE-PIK3CA-C420R (item #116457). I performed mini- and midi preparations and verified plamid production with a...

02 October 2019 4,078 2 View

Faecal sample storage: which one is better, RNAlater or ethanol 95%?

I want to isolate DNA and RNA from feline faecal samples in order to determine which pathogens (viruses, bacteria, fungi, parasites...) are present in the samples. The isolation will not be...

22 September 2019 3,029 4 View

Does anybody know the name of this plant from the Canary Islands?

15 April 2019 1,244 6 View

Any data regarding Calcium levels in glioma cells cytoplasm after adenovirus infection?

We are performing experiments on glioma cells. After infection of a cell line (U251), we see that Calcium levels increas even using a mock virus. Any reference that could explain this? Maybe...

03 March 2019 7,772 1 View

How do I choose contrasts in SPM8?

I am learning how to use SPM for PET and PET-MR analysis, but I haven't found any great resources that explain how contrasts work. I have a series of PET images where our hypothesis argues that...

24 February 2021 1,534 2 View

Are there methods for automatically mapping floodplains, fans and terraces?

I want to map these river geoforms with as little subjectivity as possible. I have a high resolution DEM generated by drone data.

22 February 2021 7,800 3 View

How to use Remote sensing and GIS applications for mapping the available medicinal plants within the locality/municipality?

Hello, I'm very interested in mapping and managing the conservation of the Philippine traditional medicinal plants, in which I think is very helpful in the field of medicine for further analysis...

21 February 2021 374 5 View

Plasmid amplification in S2 cell?

When co-transfect S2 cell with selection vector (pCoHygro or pCoBlast ) to make a stable cell line, does the gene integrate into the genome or just like E.coli? How do the plasmids amplify within...

09 February 2021 5,423 1 View

What are the limits of determining metastability in the brain?

What are temporal limits in determining brain metastability? In other words, how long does the minimum length of the scan segment using fMRI have to be for the result to be of any value? I'm...

07 February 2021 2,380 2 View

Are the hyperspectral image datasets which are publicly available at ehu.eus website atmospherically and geometrically correct?

I've come across a couple of publicly available hyperspectral image datasets from the website http://www.ehu.eus/ccwintco/index.php/Hyperspectral_Remote_Sensing_Scenes Now, my concern is...

05 February 2021 3,550 1 View

Apart from MOFA what other software is available for analysis of a multi-omic study?

Study bringing together genomics, proteomics etc together

02 February 2021 2,856 2 View

I'm trying to do pixel-wise correlation comparison of two datasets? Any available procedure or codes to perform this?

I'm trying to do pixel-wise correlation comparison of two datasets? Any standard procedure or codes to perform this? Like Correlation Coefficient map and RMSE map of a particular region.

31 January 2021 2,959 8 View

How can i improve my DNA purity for whole genome sequence that has high 260/230 ratio ?

I am performing whole genome sequence for gram negative acinetobacter baumannii using two platforms illumina miswq and oxford nanopore. Thus i require a very high yield and high purity. I am using...

31 January 2021 355 2 View

Is there any alternative to BUSCO for searching orthologues within genomes available?

I have a need to search for single-copy conservative orthologs within several plant genomes and used BUSCO tool for that. However, I found that the results produced by different versions on the...

29 January 2021 7,764 6 View