Laia Closa Gil

6 Questions 6 Answers 0 Followers

Questions related from Laia Closa Gil

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

26 February 2021 7,489 3 View

Agarose gel purification pre MinION sequencing?

Dear all, we performed an agarose gel purification with MinElute kit (QIAGEN) to extract bands between 3500 bp and 6000 bp for further sequencing with MinION. After the purification process,...

17 February 2021 5,525 1 View

Why NK cells that cause GraftversusLeukemia cannot cause GraftversusHostDisease?

I would like to know why an NK cell that causes a GraftVersusLeukemia effect against tumor cells does not attack the healthy cells of the receptor in a haploidenic transplant (causing graft versus...

13 June 2018 5,452 5 View

The best basecaller for Nanopore sequencing?

I’m working with high polymorphic target genes and I need as much accuracy as possible to be able to create my own references. If I want to use 1D2 reads, will be Albacore the only software...

16 March 2018 4,868 4 View

Average coverage measurement after mapping in CLC Genomics

Anyone knows if is it possible to measure the average coverage of my target locus after mapping my reads from the sequencer against genome? I'm using CLC Genomics Workbench 9 Regards

30 June 2017 8,967 2 View

CLC Bio: Is it possible to add the "Find binding sites and create fragments" tool in a CLC Bio Workflow?

Hi, Does anyone know if it's possible to add this tool "Find binding sites and create fragments" in a workflow? I'm working with CLC Genomics Workbench 9.5.1 and the tools i'm able to add in the...

24 April 2017 632 1 View