Hello
what is the average coefficient for changing wet weight of E.coli cells to dry weight?
and what is the average weight of each OD for E.Coli cells?
Thanks in advance.
A good rule of thumb (at least for bacterial cell mass) is to assume the dry weight to be 20 % of the respective wet massof cells. Half of the dry weight consists of protein, so protein mass is about 10% of the wet mass (provided there is no excess leftover medium in between the harvested cell mass). Moreover, I find it quite consistent to assume 1 liter of a standard bacterial culture of OD=1 to be equal to 1 g wet mass, and one bacterial cell with a volume of 1 µm^3 having the same (wet) mass as the same volume of water, which is 1 pg. Of course, this depends on the actual bacterium, growth conditions and the wave length you use for your OD (I usually use 600 nm), but I would expect it to fit within afactor of 2.
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