Can you help me with design of PCR amplification of long GC-rich regions?

Hi everyone, I was wondering if anyone has a solution for the amplification of long GC-regions using PCR? I want to isolate ~5kb long sequence from mouse genome that is unfortunately rich in...

24 August 2020 4,035 3 View

Do you know about any questionnaires for children measuring their attitudes to science and scientists?

I am doing a qualitative study on children's attitudes to science and scientists before and after our science education intervention, we deliver to primary schools. I'd like to include a...

27 January 2020 8,001 3 View

Can anyone recommend a corpus of research articles written by English native speakers?

I would like to use it as a reference corpus. I know there is a SERAC corpus, but it does not seem to be available for public.

01 April 2019 5,612 6 View

Illumina NextSeq multiplexing 384 samples per run? Barcodes?

Hello, I would like to ask you if you have any experience with multiplexing of 384 samples per run on Illumina NextSeq. We are using SNP assay designed by Roche = SeqCap EZ Choice library with...

05 December 2018 4,041 3 View

Can I use Tris/HCl instead to Bis-Tris in the gradient elfo with the same working pH and MES buffer?

I try a new method for chloroplasts analysis and its my first experiment with gradient electrophoresis for membrane proteins. In the protocol is Bis-Tris used, in lab we have Tris-HCl, the working...

04 December 2017 4,831 3 View

How to report results for generalised linear mixed model with binomial distribution?

Hello, I am currently working on the data analysis for my MSc. project comparing probability of occurrence of a species between two different habitats using presence - absence data. Due to the...

29 October 2017 8,273 5 View

Mapping ChiP-seq data on HiC data?

Hello, I am a bit new to the ChiP-seq data analysis and I need to map them onto HiC data, is there any tool to do that or visualize that somehow? Either online or using R-bioconductor? Any...

22 October 2017 8,782 1 View

Any database/online dictionary showing clearly morphemes and syllables (division)?

Hello everyone. I am urgently looking for a database or dictionary that shows already fragmented words - fragmented into morphemes and syllables as well (not pronounciation). I need it for a...

16 September 2017 8,583 1 View

Characterization for human melanoma BRO cell line?

30 April 2017 8,515 3 View

How to remove polysaccharides from protein samples?

Good afternoon, I try to extract cell wall proteins from green macroalgae, I have tried different protocols and one of them included step of EGTA extraction, however this fraction is jelly like,...

08 February 2017 1,271 2 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

What software programs can directly simulate Additive Manufacturing Directed Energy Deposition (DED)?

I understand there are a lot of software programs that can simulate DED by going through specific modeling, simulation, and commands. For me, that was time consuming and results were not accurate....

24 February 2021 4,428 3 View

I am looking for a simple software program to model 3D chemical bonds. The purpose is to generate high-quality graphical abstracts. Any suggestion?

Hello everyone: I am looking for a simple software program to model chemical bonds. The purpose is to generate a high-quality graphical abstract for a top leading journal in the field of...

23 February 2021 8,012 3 View

How to estimate 2SLS with different instruments for different endogenous variables?

To be specific, I want to conduct an IV regression in stata with the following three equations. EQ1: Y1 ~Y2 Y3 X EQ2: Y2 ~ Z1 EQ3: Y3 ~ Z2 Both Y2 and Y3 are endogenous. However, Z1 and Z2 are...

23 February 2021 6,266 3 View

Are there methods for automatically mapping floodplains, fans and terraces?

I want to map these river geoforms with as little subjectivity as possible. I have a high resolution DEM generated by drone data.

22 February 2021 7,800 3 View

How to constrain genetic algorithm input u?

My real system (buck converter) can only take an input of 0 to 1 (duty ratio) and I need to constrain the system so the controller action keeps within this bounds. How can I do this? The code for...

22 February 2021 2,125 3 View

Are there packages (R or Python) for simple models (bucket?) to assess soil water content?

Hi everyone. I am researching droguht effects on soil water drought. I am looking for already implemented functions in R or Python to estimate soil water content from precipitation,...

21 February 2021 2,084 1 View

Which is the best software or programming language (user friendly) for analysing data?

I have a basic knowledge on Python, R and MATLAB in analysing data. Among those, which is the best and user friendly? Which one should I learn deeply to analyse data?N:B: I want to learn data...

20 February 2021 3,950 15 View

Rearranging abbreviation list in LaTeX ?

Hi, I'm trying to rearrange an abbreviation list for a manuscript such that it would be listed in alphabetical order. Is there a function that does this automatically or is manual my only option?...

18 February 2021 4,870 2 View