METHOD: What method to use for transient gene silencing in Cucumis sativus ?

I'm planning to silence candidate genes in the root system of C. sativus and viral vectors seem scarce in Cucurbitaceae and with low efficacy. Therefore, I'm looking for people who have experience...

06 December 2020 1,716 1 View

How to define receptor atoms in MOE?

How can a set/define atoms as being part of the receptor for docking in MOE? I have a covalently bonded ligand-receptor complex, and I would like to perform a docking experiment with the complex...

18 October 2020 9,688 2 View

Is someone used a Single or double point method for protein intrinsic viscosity determination?

Dear all, I am wondering if someone uses a single point or double point method to determine the intrinsic viscosity of their proteins. These methods are usually developed for polymers and does...

06 August 2020 6,227 5 View

Dynamic light scattering instruments for several conditions in parallel?

Hello, Our old but not to old DynaPro Plate reader I does not work anymore and Wyatt does not want to investigate the problem as the instrument is 10 years old. We have the money to pay them but...

14 December 2019 6,735 3 View

Why DHB is a reducing agent while sDHB matrix is not a reducing agent?

Hello everyone, My question is the next : Why the DHB matrix is a reducing agent while sDHB matrix is not a reducing agent? In this article it is written that sDHB is a non reducing agent Fast...

19 October 2017 190 3 View

What is the the most validated measure of Sluggish cognitive tempo (SCT)/Concentration deficit disorder (CDD) for 6-12 YO?

What is the the most validated measure of Sluggish cognitive tempo (SCT)/Concentration deficit disorder (CDD) for 6-12 YO? Ideally a questionnaire with a parent and teacher report version

08 February 2017 6,163 1 View

Why do cells round up upon DCFDA (ROS-staining)?

25 November 2014 4,325 6 View

Do you know any good french corpora for named entity detection?

I'm looking for a french corpus tagged with named entities in order to train supervised machine learning. Can you help me to find one?

06 March 2014 7,268 3 View

How can I get the specific RNA expression of an alternative transcript of a gene of interest?

04 November 2013 9,908 6 View

What tools do you use for illustrating your articles?

Each time I write an article, I have to struggle with the problem of the illustrations. I can often see pretty nice figures in articles, more especially in reviews, which makes me think that my...

02 June 2013 3,473 20 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

How can you bring two structures together to create an adhesive bond in LAMMPS?

I have created an Ice 1h crystalline structure and an Aluminium substrate structure and equilibrated both at 250K. Now I need to bring them together in a way that an adhesive bond is created....

01 March 2021 3,325 2 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

What is the suitable range of Sulfur content and Viscosity of Heavy fluid Oil that used in Kiln?

What is the disadvantages of high sulfur content to the ceramic burning process? and what is the effect of outranged viscosity?

24 February 2021 8,219 2 View

I am looking for a simple software program to model 3D chemical bonds. The purpose is to generate high-quality graphical abstracts. Any suggestion?

Hello everyone: I am looking for a simple software program to model chemical bonds. The purpose is to generate a high-quality graphical abstract for a top leading journal in the field of...

23 February 2021 8,012 3 View

How to interpret this FTIR spectrum of pyrolyzed hydrochar?

20 February 2021 2,840 3 View

What are the potential shell well and atomic solvation parameters for the boron atom?

I have a protein-ligand docking project. The ligand contains boron atoms that are not detected by Autodock software. To solve this problem, it is necessary to introduce the boron atom to...

19 February 2021 1,236 1 View

Can bio-decomposer be a successful alternative to reduce residue burning?

To maintain the proper crop plan most of the times there is not enough time to make the field ready for the next crop. In this particular context , can bio-decompser be a solution? What about...

16 February 2021 7,068 24 View