The sample of ssDNA & protein in Tris buffer has a small peak at the area, and no obvious peak at the fingerprint region. What is small peak represent? No observable peaks at fingerprint region means my sample is too dilute?
Thanks in advance.
Hi Zul, have you run the tris buffer as background and subtracted it from sample? What is protein and DNA concentration in your sample?
Yes, the big peak at both ends of the graph is similar to the buffer. I noticed the small peak only observable in the protein-DNA mixture, however there are hardly any reference about this. Most study discuss on the wavelength at the fingerprint region. My concentration for DNA is 2500 nM and the antigen is 2500 ng/ml in the final sample (100microL).
2362 cm-1 (from NIST Web Book) - possible attributions: D2CBr+ (H2CBr+) ? (shifted). But both somewhat fit to CO2. I have both bands in my coal-fire gas RESIDUAL (after-analysis) spectra, where CO2 is an essential constituent. I wonder if they exactly come from some kind of "surplus" CO2.
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