How to interpret Generalised Linear Model output from SPSS?

I am trying to run an ordinal logistic regression using Generalised Linear Model from SPSS. There variables are A,B,C,D, and a moderation variable A*C. Variables A and D are continuous variables...

02 December 2020 5,159 3 View

How to use TransDecoder for transcriptome analysis?

I am searching for new antimicrobial peptides and I don't have many skills in bioinformatics, so I want to know how to use these software packages to identify candidate coding regions within...

01 December 2020 4,632 3 View

Case-based review vs Case report?

Hello, I have recently stumbled upon a couple of articles consisting of 'case-based review' as the title of their publication. May I ask how different is a case-based review compared to a case...

29 November 2020 8,732 2 View

How to deal with a Solver Pivot Error in Ansys (piezoeletric sensors simulation)?

I am trying to simulate the behaviour of a Piezoelectric sensor, positioned behind a plate subjected to ad impact and consequential oscillations. I have set a transient analysis and associate the...

17 November 2020 3,377 3 View

It is possible to model piezo electric behaviour with Coupled Field Transient tool?

I am trying to model a piezoelectric sensor attached to an aluminum thin plate. I know that it is possible to model a PZT using Mechanical APDL, selecting the proper elements, such as PLANE13,...

13 October 2020 7,915 11 View

How to save displacement output derived in Ansys/Autodyn and use it as input in other environment?

I conducted an analysis in autodyn saving at gauges points the displacements along z. Now, I would need to use this output data as input for analysis in the static structural environment. Since I...

15 September 2020 5,201 3 View

What if the abbreviations of two technical terms in the manuscript are the same?

I'm wrting an article, there are two terms "Carboxymethylcellulose" and "Critical Micelle Concentratio" both have the abbreviations of "CMC". How should i distinguish them?

23 June 2020 2,115 5 View

Why pulse discharge curves (capacity retention vs cycle number) is step-like?

the lithium-ion batteries (graphite||LiCoO2) charge at 500mA to 4.2 V and pulse disharge at 3A (discharge 5S, then rest 10S )to 3V. The curves (capcity retention vs.cycle number) is step -like....

02 January 2020 8,823 1 View

Has anyone observed thermal-induced twin boundary migration?

As we know, due to the tendency to decrease the overall Gibbs free energy, grain boundaries in crystalline materials are subjected to thermal-induced migration, either through a diffusive flux of...

23 December 2019 3,272 2 View

Is Cataglyphis genus present in the Arabian Peninsula?

I found contradictory information about this genus and I would like to understand its correct geographical range.

13 December 2019 8,348 8 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

Is it possible to have two luminescence bands of the same peak position but with different intensities in single spectrum??

Hi! If it is possible to have two luminescence bands of the same peak position in a spectrum, what is the reason behind formation of two separate bands instead of one? This phenomenon is related...

26 February 2021 4,963 5 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View