It is necessary to separate the serum lipoproteins, proteins are well separated at 180 volts for 30 minutes. I will be very grateful for the answer)
Is it possible to store the pvdf membrane after applying a mixture of peroxide and luminol to it?
17 April 2023 2,677 1 View
DNA is about 8kb, cells: XL1-blue, selection: ampicillin, PCR screening of clones: Encyclo Plus PCR Kit (Evrogen), DNA exstraction: Plasmid Miniprep (Evrogen). Site-directed mutagenesis per...
26 May 2021 7,063 8 View
The point is, when deposited on the heated substrate, the atomic layer (Silicon) is much smaller than when deposited on the substrate at room temperature (all other conditions were the same, the...
24 June 2018 6,033 3 View
Hello! Does anyone know about speed flying from a Taylor cone of droplets? How to calculate it? We use horizontal electrospinning.
22 January 2017 7,480 3 View
The fruit of Punica granatum is considered to be berry-like (although it was referred as irregularly dehiscent capsule in Roth 1977 and other sources). But P. granatum is completely cultivated...
21 November 2016 1,374 8 View
We found a great amount of pollen in Polygonatum flowers that are buzz-pollinated (ca 30 000), and a little lower pollen amount in the related species of the Convallariaceae, but we do not know...
28 May 2016 7,821 3 View
We have observed that Iris flower (each of three meranthia) is only once visited by bees for nectar foraging, the other visits are prohibited by negative visual or smell attraction of the...
04 February 2016 524 20 View
In my RT-qPCR the same set of primers gives different Ct values in the whole cell extract for wt and mutant strain in budding yeast. The difference is more than 5 cycles, up to no signal at all....
13 July 2015 3,121 5 View
I am interested in the differences in the structure and life cycle of these cell lines.
16 July 2013 9,514 0 View
I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....
31 July 2024 3,756 0 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View
hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...
16 July 2024 9,044 10 View
In many experiments where HK-2 cells were stimulated with TGF-β at 10 ng/mL for 48 hours, I observed that even in the control group, the baseline expression level of fibrosis proteins like FN1 and...
15 July 2024 1,929 2 View
Hello everyone. I am working on my LV concentration porject, I use 500Kda hollowfiber to do it. The HCP and BSA removal rate is nearly 80%, but last time, I failed to run it. The HCP hasn't been...
11 July 2024 5,380 0 View
Hello, So I have mouse brain tissue MS data from one of my predecessors and I have human serum data from the hospital that I collaborate with. We are trying to find markers for the diagnosis of...
11 July 2024 8,484 0 View
I seed my cell (KG1a )in opti-mem media for transfection (without lipofectamine as control ) and after 2 hours I add FBS 10 ul in each wells all my cells after 24h in control wells or with...
07 July 2024 407 0 View
Hi all, I would like to know if it is possible to use Bovine Serum Albumin (BSA) in my blocking solution for immunofluorescence muscle fiber typing. The primary antibodies' host is mouse. The...
07 July 2024 7,967 3 View
I have seen some papers that differentiate C2C12 myoblasts into myotubes by changing the growth medium that contains 10%FBS by a medium containing 2%Horse serum/calf serum + 1% NEAA. What's the...
04 July 2024 4,732 1 View
I have tried to optimize the serum free dmem which is the best but i am afraid the secretory profile has too much variations.
03 July 2024 4,044 3 View