I am looking to gently shake a 96 well plate with 100uL of cell suspension per well to keep the cells from settling on the bottom.
The Graph below shows an example of an amplification curve that shows an overshoot in fluorescence. I am using SYBR Green, BST polymerase, and 6 LAMP primers in the mixture and amplifying at 65...
14 July 2024 1,483 0 View
Before entering a project, social license and consent from the community of the said area is very crucical as it determined whether the projects is accepted and viable to the community and...
14 July 2024 5,319 0 View
I'm evaluating the reproductive performance of quails. I have the fertility, and hatchability percentages for six different sire groups and would like to determine the significant difference...
14 July 2024 2,203 5 View
I have been trying to install PowerMarker on my Windows 10 PC but it is not working. One of the requirements for PowerMarker installation is MS NET Framework 1.1.4322 condition, however, Windows...
07 June 2024 1,553 1 View
In this presence age, AI is being used in many areas of human endeavour. Be that as it may, can human creativity, empathy, and complex problem-solving abilities be replaced with AI in any field?
04 June 2024 7,327 10 View
I am looking for for mentor in medicinal or organic chemistry to help guide me in my research and career development. I am also open to collaborating with researchers who share my interest. If...
26 May 2024 5,186 2 View
A researcher always have plainful, instead of joyful, experience when they reflecting of their action taken. I, as an autoethnographer, came across of this journey, and the result is...
19 May 2024 7,347 2 View
I cannot get the Raman spectrum for carbon based materials like graphene in Raman spectroscopy of 785 nm . What can be done for obtaining the proper Raman peaks for carbon based materials in 785...
15 May 2024 3,410 2 View
am working on a seminar topic
09 May 2024 9,788 0 View
Yes, we know the roles and responsibilities of the Holy Spirit, but I want to go a little deeper in my thesis or even research on the Holy Spirit, can anyone help me?
14 April 2024 9,636 8 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...
07 August 2024 2,283 2 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
I have been performing short phagocytosis experiments using 96-round bottom, tissue culture treated, well plates (Falcon, 353077). I culture the cells with the phagocytic cargo for 2-4hrs, wash...
04 August 2024 5,228 4 View
In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
01 August 2024 4,403 4 View
Dear researchers, Today, we found this object in one of the wells of a 96wp culture plate. The plate contained MEC-1 cells and GelTrex. I am quite curious what this is (because it's not cells). Do...
01 August 2024 2,250 2 View
Does anybody know of entomological Journals without page charges that would publish an extensive checklist (Hong Kong Aculeates wasps) covering 350 species (of which more than half are new to Hong...
01 August 2024 6,134 2 View
I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....
31 July 2024 3,756 0 View
Hi, Kindly guide me that how many cells of HEC1A & HEC1B Cell lines should I seed for Wound healing assay and which plate type is recommended 6, 12 & 24?. Articles suggested mainly 24...
20 July 2024 4,143 2 View
I am simulating interlocking / dry stacked masonry in Abaqus. I am using a friction coefficient of 0.6 for my model as it is stated in the previous research as well as in the base paper. The...
16 July 2024 5,047 0 View