Antibacterial liquid inhibition assay (MIC) with LB medium?

Is it possible to determine the antibacterial minimum inhibitory concentration (MIC) using a liquid inhibition assay (MIC) with Lysogeny broth medium instead of Mueller-Hinton broth? If so, is...

01 February 2020 7,065 5 View

I am searching for a supplier to purchase the iCHIP or similiar device for in situ cultivation of sedimentary bacteria. Any suggestions please?

I am searching for a supplier to purchase the iCHIP or a similar device for in situ cultivation of sedimentary bacteria. I am looking at different technologies to try to culture previously...

10 November 2018 9,335 0 View

How to improve DNA yields using DNA extraction kits designed for soil or sediment samples?

10 November 2017 4,978 3 View

What are the current methodologies for growing fastidious marine bacteria in situ?

Can fastidious or even unculturable marine bacteria be grown in situ? If so, what are the current methodologies for doing so?

03 April 2017 1,493 2 View

What are the standard nutrient solid media used to culture marine bacteria?

I am looking for standard nutrient solid media recipes or protocols used to culture marine bacteria. What are the standard types of media used to culture marine bacteria (psychrophiles)? Also, in...

03 April 2017 9,568 4 View

Is the MCF12A cell line generally vulnerable to anticancer compounds?

Is it expected that MCF12A cells should show some level of cytotoxicity to anticancer compounds or drugs? If so, what could be the biochemical or physiological reason therefore?

11 December 2015 7,284 4 View

Are there commercially available fucoidanases or fucoidanase-like enzymes available for purchase?

07 August 2014 4,804 1 View

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02 March 2021 3,060 3 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

No title

28 February 2021 9,936 2 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View