Grant January

8 Questions 8 Answers 0 Followers

Questions related from Grant January

Antibacterial liquid inhibition assay (MIC) with LB medium?

Is it possible to determine the antibacterial minimum inhibitory concentration (MIC) using a liquid inhibition assay (MIC) with Lysogeny broth medium instead of Mueller-Hinton broth? If so, is...

02 February 2020 7,157 5 View

At what percentage of Marine broth + gellan gum does the media solidify?

I am conducting a comparative study trying to isolate bacteria on different concentrations of Marine broth (MB) media + agar vs MB + gellan gum/Gelzan/Gelrite. For the agar I have 10, 50, and...

11 November 2018 7,389 4 View

I am searching for a supplier to purchase the iCHIP or similiar device for in situ cultivation of sedimentary bacteria. Any suggestions please?

I am searching for a supplier to purchase the iCHIP or a similar device for in situ cultivation of sedimentary bacteria. I am looking at different technologies to try to culture previously...

11 November 2018 9,396 0 View

How to improve DNA yields using DNA extraction kits designed for soil or sediment samples?

I am trying to extract community DNA from deep-sea sediments using MP BIOMEDICALS FastDNA™ SPIN Kit for Soil. I have used starting material from 50 mg (recommended amount for marine sediments) to...

11 November 2017 5,023 3 View

What are the current methodologies for growing fastidious marine bacteria in situ?

Can fastidious or even unculturable marine bacteria be grown in situ? If so, what are the current methodologies for doing so?

04 April 2017 1,547 2 View

What are the standard nutrient solid media used to culture marine bacteria?

I am looking for standard nutrient solid media recipes or protocols used to culture marine bacteria. What are the standard types of media used to culture marine bacteria (psychrophiles)? Also, in...

04 April 2017 9,617 4 View

Is the MCF12A cell line generally vulnerable to anticancer compounds?

Is it expected that MCF12A cells should show some level of cytotoxicity to anticancer compounds or drugs? If so, what could be the biochemical or physiological reason therefore?

12 December 2015 7,338 4 View

Are there commercially available fucoidanases or fucoidanase-like enzymes available for purchase?

I am looking for an enzyme that can hydrolyse a fucoidan polysaccharide into oligosaccharide units.

08 August 2014 4,919 1 View