I am doing a study to assess the effect of Parthenolide on leukemic stem cell. to do that i have to prepare a stock solution by dissolving fifty-milligrams Parthenolide in 1 ml DMSO to give a 0.2 M stock solution and then store in 50-μl aliquots. Then, I will diluted one 50-μl aliquot of 0.2 M parthenolide stock solution with PBS to obtain concentrations of 5, 10, 25, 50, and 100 μm.Do you think the concentration of DMSO in this case is toxic to the cells and could mask the real effect of the tested drug?
Some quick calculations:
you dissolve your sample in 100%DMSO to a sample concentration of 0.2M (200,000uM). To get to 100uM you are doing a 1 in 2000 dilution. Therefore your most concentrated sample would be in 0.05% DMSO. That is not too high and will not interfere. However, if you further dilute this is PBS it will then have a different DMSO concentrations in the other dilutions. You do not want to introduce another variable so dilute in PBS containing 0.05% DMSO to keep the DMSO concentration constant.
1. If DMSO side-effects are a concern, one could dissolve parthenolide in absolute/100% ethanol (http://www.enzolifesciences.com/BML-T113/parthenolide/) (Suvannasankha, A, et al. 2008. Clin Cancer Res 14:1814-1822).
2. A water soluble parthenolide analog is also available (Shanmugam R et al, 2011. Int J Cancer 128: 2481-2494. PMID: 20669221)
I have a question ;
I have the Parthenolide powder 250 mg now, and I want to prepare it for in vivo study (40mg/kg) .. How to prepare this, and how much I should use DMSO.. I tried ut before and notice that up to 0.2% not toxic
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