Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Plant research: Watering uniformity/consistency. How important is it?

Trying to gather insight from an unresolved discussion with a colleague here, and either the available literature surrounding this topic and/or my keywords in search of are lacking. Context:...

21 February 2021 6,389 3 View

How to get rough estimation of the concentrations of Oxygen and moisture inside the glove box?

Dear colleagues, I was just wondering if you can suggest a good method to get a rough estimation to the concentration of O2 and H2O inside the glove box as unfortunately, our glove box doesn't...

17 February 2021 1,012 3 View

Onset of Photo Oxidation in Polymers?

Hi! I am looking for some specific information in regards to the minimum amount of UV intensity required to begin the photo degradation process in polymers. I understand each polymer is...

15 February 2021 7,703 4 View

Are you looking for a quality online conference on teaching and learning technology?

This large international conference will be held online this year due to COVID. I am submitting a paper to the Technology Enhanced Language Learning track (8). It is about a topic I have never...

15 February 2021 1,975 15 View

How do you calculate a discrimination index for polytomous data?

Hello, I'm currently running a PCA on a polytomous data set (5 point Likert). I want to create a discrimination index to narrow down my items before running the PCA. After looking around, I'm...

13 February 2021 3,517 2 View

Regarding immunology, transmissivity, herd immunity and vaccines in Sars CoV-2?

This is a follow-up on an earlier question regarding mechanistics of vaccines: why current vaccines don't block transmission. In summary, they don't engage mucosal immune cells to produce IgA....

08 February 2021 8,321 3 View

Which tropical country is the most easy for obtaining research permit (for biological research)?

Dear all, High beurocracy hihly complicate field ecolocial and evolutionary research nowadays. Nearly all tropical countries (maybe all?) requires research permit for performitng research and in...

04 February 2021 3,672 6 View

Can I polymerize NIPPAm with PEGmethacrylate or only with PEGacrylate?

I want to perform the synthesis of a hydrogel and I want to know if I can polymerize NIPPAm with PEGmethacrylate or only with PEGacrylate?

02 February 2021 2,106 6 View

What is the maximum storage time for samples used in soil enzyme assays?

01 February 2021 4,616 3 View

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01 January 2021 5,831 1 View

How are brain and mind different ?

Mind is the medium through which the world civilization was developed and any goal can be achieved.

25 December 2020 6,855 27 View

Can a Type I Civilization (Kardashev Scale) develop an Artificial Intelligence of Type II Civilization?

According to Kardashev Scale, can a Type I Civilization be able to develop artificially intelligent system that surpasses it's own intelligence to match the intelligence of higher civilizations in...

10 October 2020 2,161 8 View

Is artificial intelligence (AI) dangerous for the existance of human civilization?

At present, AI technology is reducing the human tasks and replacing the persons from their workplace. In future, it may completely replace the human in all the sectors.

26 April 2020 7,087 27 View

What is the feminism architecture?

The question discusses how architecture deals with feminism in terms of human history, through the time of Greek civilization, forms of columns, and the appearance of the Christian religion, in...

31 December 2019 2,465 2 View

What do you think, Aryans were indigenous people of India or invaders?

I viewed Aryans as True indigenous people of India. One of the early name of India i.e.' Aryavart'was on the name of Aryans. But some anthropologists and social scientists take them as invaders...

08 September 2019 6,195 2 View

Can we consider that the earth is a prison for humans?

We can direct this question to astrophysics scientists, theologians, philosophers, scientists thought and civilizations! why? Astronomy shows that the universe is very wide and the distances...

29 July 2019 421 29 View

Why is it morally permissible to eat animal C, and not animal D?

This is NOT a question about religious prohibitions or health considerations. For this issue, what is the fundamental moral principle that would be acceptable to most people from diverse...

05 June 2019 4,652 1 View

No title

05 June 2019 6,330 11 View

Is capturing the image of a black-hole socially important at all?

What would be the social implications of this new discovery? Given the most urgent problems humanity faces right now--international militant terrorism, refugee crisis, rising of the right-wing...

03 April 2019 9,796 6 View