Dear friends,
I hope this message finds you well.
I am seeking your assistance regarding the growth of Candida albicans ATCC MYA-1023 in 96-well microplates using RPMI 1640 medium (powder, Thermo Fisher, Cat. No. 31800022) supplemented with MOPS (final concentration 0.165 M). The initial inoculum was 1 × 10⁷ CFU/mL.
After 48 h of incubation at 35 °C (200 µL per well, static, flat-bottom 96-well plates), the RPMI 1/1000 dilution showed the highest OD₅₃₀/₆₀₀, approximately 0.21.
We were expecting OD₅₃₀ values in the range of ~1–1.5 after 48 h, but the readings only reached 0.21. Could you please advise on potential reasons for this discrepancy or suggest troubleshooting steps?
For your reference, please find attached the supplementary data. Thank you very much for your support.
Kind regards, Sara
IT'S glucose. I think RPMI has lower glucose (2g/L). Enriched media usually have 20g/L of glucose.
You need either a YPD overnight culture, followed by RPMI with higher inoculation, or simply add glucose or FBS to RPMI.
whatever suits your experiment
Hi. Maybe Candida is growing in clumps as hyphae. That would look like there is no growth in the wells of the plates.
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