Hi all

I am trying to assay for beta-hexosaminidase release with (

4-Methylumbelliferyl N-acetyl-β-D-glucosaminide β-N-acetylhexosaminidase substrate)

for HEK-293 cells expressing Dectin-1. When I treat the cells with Ionomycine which is supposed to trigger lysosomal exocytosis and we expect to see beta-hexosaminidase release, there is no fluorescence reported compared to non-ionomycin treated cells.

Could you please help me to solve my problem

Thanks