Making a primer
Hi,primer3 http://biotools.umassmed.edu/bioapps/primer3_www.cgi
sigma oligoarchitech is wonderful
Good job
thank you brother and sister
hi guys, i am convious with this concept, please help 1) what' s exactly cause expression of protein in spesific tissue on plant? 2) I am doing research in promoter activity right now,my promoter...
08 September 2017 1,442 0 View
I try to load DNA plasmid in Wheel gel electrophoration, but the DNA cannot load directly and brustle. is it any correlation with ethanol remaining in my DNA plasmid after isolastion?
08 September 2017 9,747 5 View
Hi, i doing my research to ligate my insert to vektor pBI121. i used 2 enzim for dygest vektor both of them is hindIII and BamHI and I continue with blunt end them both.. i do ligate for several...
01 January 1970 8,928 4 View
this is my first experience in overlap PCR, i must attach restriction enzim in my primer, and that'sprimer will use and ligase at plasmid. but the plasmid and my gen interest didnt have suit...
01 January 1970 7,515 6 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
Please can anyone support with the survey questions based on RQ measures and propose how to do it in FMCG industry and include as well the role of brand equity Thanks
06 August 2024 949 0 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
How to design VN primer to attach with universal reverse primer
05 August 2024 2,116 3 View
Hello! I have this scale which had 10 items initially. I had to remove items 8 and 10 because they correlated negatively with the scale, and then I removed item 9 because Cronbach's alpha and...
01 August 2024 4,606 7 View
It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...
01 August 2024 4,673 4 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View
Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...
30 July 2024 841 2 View
In STATISTICA 13.3, I used the Experimental Design Builder under the Design of Experiments tab to generate a design matrix for an asymmetrical factorial design (1443) with 12 experiments. However,...
29 July 2024 4,155 2 View
Employing a pragmatic inquiry research design, looking for published research using this method, employing qualitative research data collection methods of semi-structured interview and focus...
28 July 2024 540 2 View
