Agus Eka Prasetyo

5 Questions 7 Answers 0 Followers

Questions related from Agus Eka Prasetyo

Why DNA plasmid have to dry after wash by ethanol??

I try to load DNA plasmid in Wheel gel electrophoration, but the DNA cannot load directly and brustle. is it any correlation with ethanol remaining in my DNA plasmid after isolastion?

09 September 2017 9,730 5 View

Protein expression in plant - what' s exactly cause expression of protein in spesific tissue on plant?

hi guys, i am convious with this concept, please help 1) what' s exactly cause expression of protein in spesific tissue on plant? 2) I am doing research in promoter activity right now,my promoter...

09 September 2017 1,425 0 View

[ ask ] what' s the free online website for creating primer for free except NCBI?

Making a primer

10 October 2016 7,014 3 View

[ help- overlap PCR ]

this is my first experience in overlap PCR, i must attach restriction enzim in my primer, and that'sprimer will use and ligase at plasmid. but the plasmid and my gen interest didnt have suit...

01 January 1970 7,498 6 View

Blunt end ligation problem, can anyone help?

Hi, i doing my research to ligate my insert to vektor pBI121. i used 2 enzim for dygest vektor both of them is hindIII and BamHI and I continue with blunt end them both.. i do ligate for several...

01 January 1970 8,910 4 View