What is meant by bimodal gene expression in real time PCR?

Please explain the bimodal pattern of expression in real time PCR. What is meant by it?

09 October 2017 1,833 5 View

Can we do comparitive proteomics using the normal SDS-PAGE, only through one dimension separation.....any body please help?

I am using an un inoculated leaf sample and inoculated leaf sample with Phytophthora. So I want to see the deferentially expressed proteins in the inoculated sample when compared to the un...

02 March 2016 6,095 4 View

Does anybody have any idea about the availability of free software for gene normalization in qPCR experiments?

The standard software Genorm and Normfinder I could not access freely. Kindly tell me if there is any free software and the latest version of it if available. Please its very urgent....PLS HELP

08 September 2015 4,965 5 View

The resistant source is a non host plant for a particular pathogen. Can non host plant be used in breeding programme?

can this resistance from a non host plant be a durable one? can trangenics be created out of resistant source if the source is a non host

02 March 2015 9,368 4 View

Is the change from vegetatively propagated plants that show resistance, to showing susceptiblity when the plants are seedlings due to segregation?

01 February 2015 2,699 17 View

is there any difference between pathogenecity genes or pathogenecity related genes and virulence genes?

pls very urgent

05 June 2014 320 0 View

What is the best tool for doing multiple sequence alignment for a particular gene and also drawing the phylogenetic tree

I have some defence gene sequences from Piper sp which was induced upon inoculation with Phytophthora. These sequences were identified via Transcriptome sequencing. I would like to know how to do...

01 January 1970 9,659 26 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View

Statistical methods for comparing gene expression curves?

Hi all, I have generated two gene expression curves by measuring bioluminescence every 10 mins for 12 hrs. These curves tend to overlap at at some time points, while a difference can be seen at...

02 February 2021 2,613 7 View

Help needed: How to map AA, AG, and GG genotypes on to polymorphism information?

Dear Colleagues, I am new to genes and genotyping and I need help. I am trying to figure our how AA, AG, and GG genotypes map on to A1 for DRD2/ANKK1 Taq1A polymorphism(rs1800497) and Val or MET...

02 February 2021 3,861 3 View

New knockout bacterial strain: Can't electroporate it?

I made a new bacterial strain with an unmarked deletion using 2 step homologous recombination (with sacB counterselection). The full protocol is given in the link at the bottom. The protocol...

01 January 2021 1,580 4 View

Can you advise reference publications/standards on characterizing plant genetics research and development activities, and the outputs it generates?

I am doing semantic analysis of science and grey literature publications in the field of plant genetics and breeding. I have a standard dictionary but I am not sure if it captures the state of art...

31 December 2020 5,871 4 View

What is the role of fresh cow dung in sprouting in stem cutting propagation?

In my personal experience I have find the higher rate of sprouting when fresh cow dung is applied on the top side of cutting what might be its reason.

24 December 2020 4,068 6 View

What is the level of consciousness , communication and feeling in plants ?

Do you think the plant can communicate with each other, what is the level of feeling in plants?

22 December 2020 2,754 17 View

How to cut dsDNA chain with Uracil-DNA Glycosylase at the point of dU in room temperature?

18 December 2020 6,908 3 View

Why pink coloured or light red onion have more storage life than white and dark red one and smaller size one to has more than bigger one?

It is generally seen light coloured varieties has more storage life.what's relation with colour and size with storage life.

13 December 2020 634 18 View

Can LAMP distinguish the species from different genus/family?

I am usiing LAMP kit, specifically designed for liriomyza huidobrensis. I have a random samples of differet insects and LAMP seems to amplify insects from other Genus as well. Although, other...

07 December 2020 5,630 3 View