What is the best pipeline design software?

Hello i am looking for a software which can be used to design pipeline for a heating system. I need it to study the stress in pipes etc... Any software which could be tested with a free version...

28 March 2018 3,778 3 View

Concentration of Anti-17R used in breast cancer cell lines culture ?

Hello everybody! I have an anti-IL17R neutralizing antibody, the stock concentration is 0.5mg/ml , what could be the used concentration to neutralize IL17 in cells culture?

07 January 2018 5,920 3 View

Hello everyone, how to defrost human tissues stored in 10% DMSO for flow cytometry analysis?

Hi everyone, I am currently working on the analysis of T lymphocyte sub populations in breast cancer tissues using flow cytometry, currently I have frozen breast tissues in DMSO 10 % SVF,...

13 November 2017 3,486 1 View

How can we calculate the activity of SO4 in sediment

29 June 2017 4,530 2 View

How can I express total Cd in water Cd or Cd+2 why?

How can I express total Cd in water Cd or Cd+2 why

27 May 2017 3,499 4 View

What is the endpoint of pH when measuring total alkalinity?

28 March 2016 2,130 3 View

How should I go about evaluating cytoplasmic and nuclear Arginase-1 activity?

Hello! I've stained some bright field Arginase-1 Hematoxylin slides, but I'm having a hard time finding any computed histology predicates for evaluating the intensity of the cytoplasm verses the...

03 February 2021 3,039 1 View

IHC-paraffin experiment: how do you approach shopping for an antibody?

Hello everyone! I am shopping for an arginase 1 antibody to use for IHC-paraffin experiments with rat tissue. So far what I have understood is that if an antibody says it is reactive with rat,...

16 January 2021 794 3 View

Staining protocol using arginase 2 antibody and Hoechest 33342?

I need the staining protocol for staining macrophages with arginase 2 antibody and Hoechest 33342 dye. Which of the stains comes first. Hoechest 33342 is a counter stain right? The antibody was...

19 August 2020 3,648 4 View

Could anyone recommend anti-mouse-Arginase and anti-mouse NOS2 antibodies for flow?

I'd like to run flow to test expression of arginase and iNOS in mouse tissue by flow cytometry. I appreciate it, if anyone could tell the catalogs number or clone number of the antibodies which...

09 October 2018 6,050 0 View

Can someone tell me how I can extract arginase?

Hi, I want to work on the in vitro activity of arginase and I would like to know if it is possible to extract it ? What is the most suitable tissue and the most effective method ? Thank you.

09 October 2018 3,264 0 View

What is the protocol for arginase and iNOS Staining?

I am looking for an ics protocol for staining arginase1

06 July 2018 6,531 0 View

Can anyone help me to find the Tm value of a qPCR product using beacon designer software (biorad) because my licence has expired?

accession number: NM_019168.1 (arginase 2) primer F: 5'CTC-TGG-ATC-TTG-TTG-AAG3' primer R: 5'ACT-TGA-AGC-AAT-CAC-ATC3' I need the value of the melting temperature (Tm) of the product...

03 April 2018 7,579 1 View

Plateau of an enzyme inhibition?

I am doing an experiment on arginase to see if different compounds can inhibit this enzyme. I want to calculate IC50 and i reached the plateau at a inhibition of 80%. it is ok or i should increase...

02 March 2018 916 5 View

Arginase 1 detection by western blot

We are trying to detect arginase 1 from activated macrophages by western blot. We are using peritoneal mice macrophages activated for 24h with IL-4. In that condition, we have high ARG 1 activity...

05 June 2017 3,840 0 View

Is it any precise method for assessment of arginase inhibition of Leishmania by some drug compounds?

Inhibition of this enzyme activity in Leishmania is very important and could help scientists to reach suitable drugs.

13 July 2016 1,933 2 View