Arginase activity in stored organs can be measured ?

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How to purify tau protein in low temperature by tris buffer?

how to purify tau protein in low temperature by tris buffer?

16 December 2023 1,203 0 View

How i clean PD minitrap G10 column???

27 November 2023 3,077 0 View

What are thedrowbacks in meassuring thé nanoworld?

AND there Is a gold technique that Is suitible for all meassurment off nano world????

22 December 2022 8,393 3 View

Does anyone have ever used the qMAXSenTM One-Step Green RT-qPCR Kit (High RoxTM)?

Hello, Has anyone studied gene expression using the qMAXSenTM One-Step Green RT-qPCR Kit (High RoxTM), and if so, in which Real-Time PCR instrument?

12 May 2022 5,397 0 View

Why are current commercial CSP systems with thermal energy storage limited to capacities below 20h?

Increasing the capacity of the thermal energy storage system beyond 20 hours seems to pose an economic problem, as the cost of the tanks increases for higher capacities as a result of the...

19 January 2022 2,471 8 View

How to define two reference temperature for two fluids or PCMs in ANSYS Fluent?

Hello everyone, I'm facing a problem when it comes to defining the parameters of Boussinesq approximation in Ansys Fluent. I'm simulating a fluid and a PCM with Boussinesq approximation, which...

18 September 2021 6,677 4 View

Recover virulence of Leishmania parasites ?

hello everyone The strains of parasites Leishmania wich I'm currently working with exceeded 10 passages. I would like to know if there is another way to recover their virulence, a part from...

03 April 2020 6,339 2 View

What is the best pipeline design software?

Hello i am looking for a software which can be used to design pipeline for a heating system. I need it to study the stress in pipes etc... Any software which could be tested with a free version...

28 March 2018 4,111 3 View

Concentration of Anti-17R used in breast cancer cell lines culture ?

Hello everybody! I have an anti-IL17R neutralizing antibody, the stock concentration is 0.5mg/ml , what could be the used concentration to neutralize IL17 in cells culture?

07 January 2018 5,975 3 View

Hello everyone, how to defrost human tissues stored in 10% DMSO for flow cytometry analysis?

Hi everyone, I am currently working on the analysis of T lymphocyte sub populations in breast cancer tissues using flow cytometry, currently I have frozen breast tissues in DMSO 10 % SVF,...

13 November 2017 3,540 1 View

What functional tests to perform to prove pro tumor activity of human neutrophils?

What are the best functional analyses to prove the pro tumor activity of human neutrophils after isolation from patients with hematological malignancies? We are considering assays to detect...

30 April 2021 2,381 2 View

Someone with experience using nor-NOHA?

I would like to block arginase in BMDM, both arginase 1 and 2, I thought about using nor-NOHA. Does anyone know the difference between nor-NOHa acetate and monoacetate? I accept suggestions

17 March 2021 9,654 1 View

How should I go about evaluating cytoplasmic and nuclear Arginase-1 activity?

Hello! I've stained some bright field Arginase-1 Hematoxylin slides, but I'm having a hard time finding any computed histology predicates for evaluating the intensity of the cytoplasm verses the...

03 February 2021 3,470 1 View

IHC-paraffin experiment: how do you approach shopping for an antibody?

Hello everyone! I am shopping for an arginase 1 antibody to use for IHC-paraffin experiments with rat tissue. So far what I have understood is that if an antibody says it is reactive with rat,...

16 January 2021 1,123 3 View

Staining protocol using arginase 2 antibody and Hoechest 33342?

I need the staining protocol for staining macrophages with arginase 2 antibody and Hoechest 33342 dye. Which of the stains comes first. Hoechest 33342 is a counter stain right? The antibody was...

19 August 2020 3,750 4 View

Could anyone recommend anti-mouse-Arginase and anti-mouse NOS2 antibodies for flow?

I'd like to run flow to test expression of arginase and iNOS in mouse tissue by flow cytometry. I appreciate it, if anyone could tell the catalogs number or clone number of the antibodies which...

09 October 2018 6,066 0 View

Can someone tell me how I can extract arginase?

Hi, I want to work on the in vitro activity of arginase and I would like to know if it is possible to extract it ? What is the most suitable tissue and the most effective method ? Thank you.

09 October 2018 3,409 0 View

What is the protocol for arginase and iNOS Staining?

I am looking for an ics protocol for staining arginase1

06 July 2018 6,545 0 View

Can anyone help me to find the Tm value of a qPCR product using beacon designer software (biorad) because my licence has expired?

accession number: NM_019168.1 (arginase 2) primer F: 5'CTC-TGG-ATC-TTG-TTG-AAG3' primer R: 5'ACT-TGA-AGC-AAT-CAC-ATC3' I need the value of the melting temperature (Tm) of the product...

03 April 2018 7,600 1 View

Plateau of an enzyme inhibition?

I am doing an experiment on arginase to see if different compounds can inhibit this enzyme. I want to calculate IC50 and i reached the plateau at a inhibition of 80%. it is ok or i should increase...

11 March 2018 142 3 View