Can anyone help me to find the Tm value of a qPCR product using beacon designer software (biorad) because my licence has expired?

Hélène Martin @Helene_Martin

04 April 2018 1 8K Report

accession number: NM_019168.1 (arginase 2)

primer F: 5'CTC-TGG-ATC-TTG-TTG-AAG3'

primer R: 5'ACT-TGA-AGC-AAT-CAC-ATC3'

I need the value of the melting temperature (Tm) of the product (95bp)

thank you very much for your help

Hélène

Frederic Lepretre

Hi Hélène,

it depends on the species you're working on but UCSC has some genomes and useful tools as PCR in silico that will give you a Tm and more stuffs.

fred

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