What are the best functional analyses to prove the pro tumor activity of human neutrophils after isolation from patients with hematological malignancies? We are considering assays to detect Arginase 1, ROS-production or performing co-cultures with autologous t-cells, but thus far have not found any common functional criteria for classification.
Hi Richard
I am somewhat unclear of your question. Do you want to to prove a pro-tumour activity meaning neutrophils promote tumour growth or anti-tumour activity (i.e. inhibiting tumour growth)?
Siva
Hi Muttuswamy Sivakumaran ,
I want to prove tumor-promoting activity of neutrophils as previously described in N2-polarized TANs. I found this area of research to be very heterogenous and yet without any common standards for functional assays- unlike in MDSC. So I was wondering whether somebody maybe had any suggestions or references.
Cheers
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